Soybean cultivar 943465628996

ABSTRACT

A novel soybean cultivar, designated 943465628996, is disclosed. The invention relates to the seeds of soybean cultivar 943465628996, to the plants of soybean 943465628996 and to methods for producing a soybean plant produced by crossing the cultivar 943465628996 with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar 943465628996 with another soybean cultivar.

BACKGROUND OF THE INVENTION

The present invention relates to a new and distinctive soybean cultivar,designated 943465628996. There are numerous steps in the development ofany novel, desirable plant germplasm. Plant breeding begins with theanalysis and definition of problems and weaknesses of the currentgermplasm, the establishment of program goals, and the definition ofspecific breeding objectives. The next step is selection of germplasmthat possess the traits to meet the program goals. The goal is tocombine in a single variety an improved combination of desirable traitsfrom the parental germplasm. These important traits may include higherseed yield, resistance to diseases and insects, better stems and roots,tolerance to drought and heat, and better agronomic quality.

Choice of breeding or selection methods depends on the mode of plantreproduction, the heritability of the trait(s) being improved, and thetype of cultivar used commercially (e.g., F₁ hybrid cultivar, purelinecultivar, etc.). For highly heritable traits, a choice of superiorindividual plants evaluated at a single location will be effective,whereas for traits with low heritability, selection should be based onmean values obtained from replicated evaluations of families of relatedplants. Popular selection methods commonly include pedigree selection,modified pedigree selection, mass selection, and recurrent selection.

The complexity of inheritance influences choice of the breeding method.Backcross breeding is used to transfer one or a few favorable genes fora highly heritable trait into a desirable cultivar. This approach hasbeen used extensively for breeding disease-resistant cultivars. Variousrecurrent selection techniques are used to improve quantitativelyinherited traits controlled by numerous genes. The use of recurrentselection in self-pollinating crops depends on the ease of pollination,the frequency of successful hybrids from each pollination, and thenumber of hybrid offspring from each successful cross.

Each breeding program should include a periodic, objective evaluation ofthe efficiency of the breeding procedure. Evaluation criteria varydepending on the goal and objectives, but should include gain fromselection per year based on comparisons to an appropriate standard,overall value of the advanced breeding lines, and number of successfulcultivars produced per unit of input (e.g., per year, per dollarexpended, etc.).

Promising advanced breeding lines are thoroughly tested and compared toappropriate standards in environments representative of the commercialtarget area(s) for three or more years. The best lines are candidatesfor new commercial cultivars; those still deficient in a few traits maybe used as parents to produce new populations for further selection.

These processes, which lead to the final step of marketing anddistribution, usually take from eight to 12 years from the time thefirst cross is made. Therefore, development of new cultivars is atime-consuming process that requires precise forward planning, efficientuse of resources, and a minimum of changes in direction.

A most difficult task is the identification of individuals that aregenetically superior, because for most traits the true genotypic valueis masked by other confounding plant traits or environmental factors.One method of identifying a superior plant is to observe its performancerelative to other experimental plants and to a widely grown standardcultivar. If a single observation is inconclusive, replicatedobservations provide a better estimate of its genetic worth.

The goal of plant breeding is to develop new, unique and superiorsoybean cultivars and hybrids. The breeder initially selects and crossestwo or more parental lines, followed by repeated selfing and selection,producing many new genetic combinations. The breeder can theoreticallygenerate billions of different genetic combinations via crossing,selfing and mutations. The breeder has no direct control at the cellularlevel. Therefore, two breeders will never develop the same line, or evenvery similar lines, having the same soybean traits.

Each year, the plant breeder selects the germplasm to advance to thenext generation. This germplasm is grown under unique and differentgeographical, climatic and soil conditions, and further selections arethen made, during and at the end of the growing season. The cultivarswhich are developed are unpredictable. This unpredictability is becausethe breeder's selection occurs in unique environments, with no controlat the DNA level (using conventional breeding procedures), and withmillions of different possible genetic combinations being generated. Abreeder of ordinary skill in the art cannot predict the final resultinglines he develops, except possibly in a very gross and general fashion.The same breeder cannot produce the same cultivar twice by using theexact same original parents and the same selection techniques. Thisunpredictability results in the expenditure of large amounts of researchmonies to develop superior new soybean cultivars.

The development of new soybean cultivars requires the development andselection of soybean varieties, the crossing of these varieties andselection of superior hybrid crosses. The hybrid seed is produced bymanual crosses between selected male-fertile parents or by using malesterility systems. These hybrids are selected for certain single genetraits such as pod color, flower color, pubescence color or herbicideresistance which indicate that the seed is truly a hybrid. Additionaldata on parental lines, as well as the phenotype of the hybrid,influence the breeder's decision whether to continue with the specifichybrid cross.

Pedigree breeding and recurrent selection breeding methods are used todevelop cultivars from breeding populations. Breeding programs combinedesirable traits from two or more cultivars or various broad-basedsources into breeding pools from which cultivars are developed byselfing and selection of desired phenotypes. The new cultivars areevaluated to determine which have commercial potential.

Pedigree breeding is used commonly for the improvement ofself-pollinating crops. Two parents which possess favorable,complementary traits are crossed to produce an F₁. An F₂ population isproduced by selfing one or several F₁'s. Selection of the bestindividuals may begin in the F₂ population; then, beginning in the F₃,the best individuals in the best families are selected. Replicatedtesting of families can begin in the F₄ generation to improve theeffectiveness of selection for traits with low heritability. At anadvanced stage of inbreeding (i.e., F₆ and F₇), the best lines ormixtures of phenotypically similar lines are tested for potentialrelease as new cultivars.

Mass and recurrent selections can be used to improve populations ofeither self- or cross-pollinating crops. A genetically variablepopulation of heterozygous individuals is either identified or createdby intercrossing several different parents. The best plants are selectedbased on individual superiority, outstanding progeny, or excellentcombining ability. The selected plants are intercrossed to produce a newpopulation in which further cycles of selection are continued.

Backcross breeding has been used to transfer genes for a simplyinherited, highly heritable trait into a desirable homozygous cultivaror inbred line which is the recurrent parent. The source of the trait tobe transferred is called the donor parent. The resulting plant isexpected to have the attributes of the recurrent parent (e.g., cultivar)and the desirable trait transferred from the donor parent. After theinitial cross, individuals possessing the phenotype of the donor parentare selected and repeatedly crossed (backcrossed) to the recurrentparent. The resulting plant is expected to have the attributes of therecurrent parent (e.g., cultivar) and the desirable trait transferredfrom the donor parent.

The single-seed descent procedure in the strict sense refers to plantinga segregating population, harvesting a sample of one seed per plant, andusing the one-seed sample to plant the next generation. When thepopulation has been advanced from the F₂ to the desired level ofinbreeding, the plants from which lines are derived will each trace todifferent F₂ individuals. The number of plants in a population declineseach generation due to failure of some seeds to germinate or some plantsto produce at least one seed. As a result, not all of the F₂ plantsoriginally sampled in the population will be represented by a progenywhen generation advance is completed.

In a multiple-seed procedure, soybean breeders commonly harvest one ormore pods from each plant in a population and thresh them together toform a bulk. Part of the bulk is used to plant the next generation andpart is put in reserve. The procedure has been referred to as modifiedsingle-seed descent or the pod-bulk technique.

The multiple-seed procedure has been used to save labor at harvest. Itis considerably faster to thresh pods with a machine than to remove oneseed from each by hand for the single-seed procedure. The multiple-seedprocedure also makes it possible to plant the same number of seeds of apopulation each generation of inbreeding. Enough seeds are harvested tomake up for those plants that did not germinate or produce seed.

Descriptions of other breeding methods that are commonly used fordifferent traits and crops can be found in one of several referencebooks (e.g., Allard, 1960; Simmonds, 1979; Sneep et al., 1979; Fehr,1987).

Proper testing should detect any major faults and establish the level ofsuperiority or improvement over current cultivars. In addition toshowing superior performance, there must be a demand for a new cultivarthat is compatible with industry standards or which creates a newmarket. The introduction of a new cultivar will incur additional coststo the seed producer, the grower, processor and consumer; for specialadvertising and marketing, altered seed and commercial productionpractices, and new product utilization. The testing preceding release ofa new cultivar should take into consideration research and developmentcosts as well as technical superiority of the final cultivar. Forseed-propagated cultivars, it must be feasible to produce seed easilyand economically.

Soybean, Glycine max (L), is an important and valuable field crop. Thus,a continuing goal of plant breeders is to develop stable, high yieldingsoybean cultivars that are agronomically sound. The reasons for thisgoal are obviously to maximize the amount of grain produced on the landused and to supply food for both animals and humans. To accomplish thisgoal, the soybean breeder must select and develop soybean plants thathave the traits that result in superior cultivars.

SUMMARY OF THE INVENTION

According to the invention, there is provided a novel soybean cultivar,designated 943465628996. This invention thus relates to the seeds ofsoybean cultivar 943465628996, to the plants of soybean 943465628996 andto methods for producing a soybean plant produced by crossing thesoybean 943465628996 with itself or another soybean line.

DEFINITIONS

In the description and tables which follow, a number of terms are used.In order to provide a clear and consistent understanding of thespecification and claims, including the scope to be given such terms,the following definitions are provided:

Maturity Date. Plants are considered mature when 95% of the pods havereached their mature color. The number of days are either calculatedfrom September 1 or from the planting date.

Seed Yield (Bushels/Acre). The yield in bushels/acre is the actual yieldof the grain at harvest.

Lodging Resistance. Lodging is rated on a scale of 1 to 5. A score of 1indicates erect plants. A score of 2.5 indicates plants are leaning at a45° angle in relation to the ground and a score of 5 indicates plantsare laying on the ground.

Phytophthora Tolerance. Tolerance to Phytophthora root rot is rated on ascale of 1 to 5, with a score of 1 being the best or highest toleranceranging down to a score of 5 which indicates the plants have notolerance to Phytophthora.

Emergence. This score indicates the ability of the seed to emerge whenplanted 3″ deep in sand and with a controlled temperature of 25° C. Thenumber of plants that emerge each day are counted. Based on this data,each genotype is given a 1 to 5 score based on its rate of emergence andpercent of emergence. A score of 1 indicates an excellent rate andpercent of emergence, an intermediate score of 2.5 indicates averageratings and a 5 score indicates a very poor rate and percent ofemergence.

Iron-Deficiency Chlorosis. Plants are scored 1 to 5 based on visualobservations. A score of 1 means no stunting of the plants or yellowingof the leaves and a score of 5 indicates the plants are dead or dyingcaused by iron-deficiency chlorosis, a score of 2.5 means plants haveintermediate health with some leaf yellowing.

Brown Stem Rot. This is a visual disease score from 1 to 5 comparing allgenotypes in a given test. The score is based on leaf symptoms ofyellowing and necrosis caused by brown stem rot. A score of 1 indicatesno symptoms. Visual scores range to a score of 5 which indicates severesymptoms of leaf yellowing and necrosis.

Shattering. The amount of pod dehiscence prior to harvest. Poddehiscence involves seeds falling from the pods to the soil. This is avisual score from 1 to 5 comparing all genotypes within a given test. Ascore of 1 means pods have not opened and no seeds have fallen out. Ascore of 2.5 indicates approximately 50% of the pods have opened, withseeds falling to the ground and a score of 5 indicates 100% of the podsare opened.

Plant Height. Plant height is taken from the top of soil to top node ofthe plant and is measured in inches.

Seed Protein Peroxidase Activity. Seed protein peroxidase activity isdefined as a chemical taxonomic technique to separate cultivars based onthe presence or absence of the peroxidase enzyme in the seed coat. Thereare two types of soybean cultivars, those having high peroxidaseactivity (dark red color) and those having low peroxidase activity (nocolor).

DETAILED DESCRIPTION OF THE INVENTION

Soybean cultivar 943465628996 has superior characteristics and wasdeveloped from the cross H13443×3HS302-12×A3313×2835×A3237/3×40-3-2. F₁and F₂ plants were advanced by a modified pedigree selection. F₃ derivedF₄ lines were selected in 1995 and 1996. In 1996 943465628996 wasentered in a yield test at 4 locations in the upper Midwest, where itplaced 1st of 41 entries.

943465628996 is a mid-maturity group III variety with resistance to theRoundup Ready™ herbicide. This variety has very high yield potentialwhen compared to lines of similar maturity. 943465628996 has excellentagronomic characteristics, including superior lodging resistance andexcellent appearance. In tests, it has beat Asgrow A3510 by 112%overall, winning at 4 of 4 locations. It has a major gene forPhytophthora resistance, Rps1C, conferring resistance to most races ofPhytophthora Root Rot.

Some of the criteria used to select in various generations include: seedyield, lodging resistance, emergence, disease tolerance, maturity, lateseason plant intactness, plant height and shattering resistance.

The cultivar has shown uniformity and stability for the traits, asdescribed in the following variety description information. It has beenself-pollinated a sufficient number of generations with carefulattention to uniformity of plant type. The line has been increased withcontinued observation for uniformity.

Soybean cultivar 943465628996 has the following morphologic and othercharacteristics (based primarily on data collected at Oxford, Ind.)

VARIETY DESCRIPTION INFORMATION 1. Seed Shape: Spherical Flattened (L/Wratio > 1.2; L/T ratio = <1.2) 2. Seed Coat Color: (Mature Seed) -Yellow 3. Seed Coat Luster: (Mature Hand Shelled Seed) - Dull 4. HilumColor: (Mature Seed) - Imperfect Black 5. Cotyledon Color: (MatureSeed) - Yellow 6. Hypocotyl Color: Light purple below cotyledons 7.Leaflet Shape: Ovate 8. Flower Color: Purple 9. Pod Color: Brown 10.Plant Pubescence Color: Gray 11. Plant Habit: Indeterminate 12. MaturityGroup: III 13. Disease Reaction: Phytophthora Rot (Phytophthoramegasperma var. sojae): Race 1 Resistant Race 2 Resistant Race 3Resistant Race 4 Susceptible Race 5 Susceptible Race 6 Resistant Race 7Resistant Race 8 Resistant Race 9 Resistant Race 10 Resistant Race 11Resistant Race 13 Resistant Race 15 Resistant Race 17 Resistant Race 21Resistant Race 23 Resistant Race 24 Resistant Soybean Cyst Nematode(Heterodera glycines): Race 3 Susceptible Race 14 Susceptible 14.Physiological Responses: Roundup Ready herbicides: Resistant 15. PlantLodging Score: 1.8

This invention is also directed to methods for producing a soybean plantby crossing a first parent soybean plant with a second parent soybeanplant, wherein the first or second soybean plant is the soybean plantfrom the line 943465628996. Further, both first and second parentsoybean plants may be from the cultivar 943465628996. Therefore, anymethods using the cultivar 943465628996 are part of this invention:selfing, backcrosses, hybrid breeding, and crosses to populations. Anyplants produced using cultivar 943465628996 as a parent are within thescope of this invention. As used herein, the term “plant” includes plantcells, plant protoplasts, plant cells of tissue culture from whichsoybean plants can be regenerated, plant calli, plant clumps, and plantcells that are intact in plants or parts of plants, such as pollen,flowers, seeds, pods, leaves, stems, and the like. Thus, another aspectof this invention is to provide for cells which upon growth anddifferentiation produce the cultivar 943465628996.

The cultivar 943465628996 is similar to A3244. While similar, there arenumerous differences including: 943465628996 has a resistance toglyphosate (Roundup Ready™) herbicide.

TABLES

In the Tables that follow, the traits and characteristics of soybeancultivar 943465628996 are compared to several competing varieties ofcommercial soybeans of similar maturity. In the table, column 1 showsthe yield in bushels/acre for the instant invention and the CompetitorVariety. Column 2 indicates the days to maturity after September 1 forthe instant invention and the Competitor Variety. Column 3 shows theplant height in inches for the instant invention and the CompetitorVariety. Column 4 indicates the plant lodging for the instant inventionand the Competitor Variety. Column 5 shows the general rating scores forthe instant invention and the Competitor Variety. Lodging and GeneralRating scores are rated 1=Best and 5=Worst.

TABLE 1 1996 YIELD DATA - LOCATIONS: IA, IL, IN & WI BU/A MAT HGT LDG GROverall Mean 54.28 34.50 33.30 1.70 2.30 Number of Locations 4 4 3 4 4943465628996 60.26 36.00 34.70 1.80 2.00 Asgrow A3134 56.65 32.00 32.001.80 2.50 Asgrow A3244 54.80 33.00 31.00 1.40 1.30 Asgrow A3510 53.5336.00 32.00 1.80 2.80

TABLE 2 1997 YIELD DATA BU/A MAT HGT LDG GR Overall Mean 56.97 27.7033.70 1.40 2.20 Number of Locations 21 20 20 17 19 Asgrow A3559 60.4429.20 34.00 1.20 1.90 Asgrow A3404 59.91 29.00 34.20 1.80 2.00 Stine3260 59.75 30.00 33.90 1.70 2.50 Asgrow A3244 59.10 26.60 32.00 1.101.60 943465628996 58.17 29.60 34.50 1.30 1.90 Pioneer 9392 57.86 30.5034.80 1.30 1.80 Asgrow A3134 57.70 27.00 31.90 1.40 2.20 Asgrow A283357.28 22.30 28.40 1.00 2.20 Asgrow AG3002 56.85 25.80 32.50 1.60 2.50Pioneer 9342 56.32 27.00 32.80 1.40 2.40 Dekalb CX313 56.31 26.30 34.301.70 2.80 Stinie 3264 55.29 30.70 33.60 1.40 2.10 Pioneer 9333 55.0325.80 30.60 1.10 2.20 Asgrow AG3301 54.83 27.40 36.00 1.60 2.50 AsgrowAG2901 53.80 24.30 34.90 1.30 2.50 Northrup King S35-F5 52.89 31.4033.20 1.30 2.80 Pioneer 9363 52.27 26.00 35.30 1.80 3.20

A deposit of the Asgrow Seed Company soybean cultivar 943465628996disclosed above and recited in the appended claims has been made withthe American Type Culture Collection (ATCC), 10801 University Boulevard,Manassas, Va. 20110. The date of deposit was May 21, 1999. The depositof 2,500 seeds were talcen from the same deposit maintained by AsgrowSeed Company since prior to the filing date of this application. Allrestrictions upon the deposit have been removed, and the deposit isintended to meet all of the requirements of 37 C.F.R §1.801-1-809. TheATCC accession number is ATCC PTA-97. The deposit will be maintained inthe depository for a period of 30 years, or 5 years after the lastrequest, or for the effective life of the patent, whichever is longer,and will be replaced as necessary during that period.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

What is claimed is:
 1. A soybean seed designated 943465628996 and havingATCC Accession No. PTA-97.
 2. A plant and its parts produced by thegrowing seed of claim
 1. 3. Pollen of the plant of claim
 2. 4. An ovuleof the plant of claim
 2. 5. A soybean plant with all physiological andmorphological characteristics of the soybean plant of claim
 2. 6. Tissueculture of the plant of claim
 2. 7. A soybean plant regenerated from thetissue culture of claim 6, wherein said regenerated soybean plant iscapable of expressing all the physiological and morphologicalcharacteristics of a soybean seed designated
 943465628996. 8. A methodfor producing a hybrid soybean seed comprising crossing a first parentsoybean plant with a second parent soybean plant and harvesting theresultant hybrid soybean seed, wherein said first or second parentsoybean plant is the soybean plant of claim
 2. 9. A hybrid seed producedby the method of claim
 8. 10. A hybrid plant and its parts produced bygrowing said hybrid soybean seed of claim
 9. 11. Seed produced from saidhybrid plant of claim 10.